Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Full article: Paracetamol: overdose induced oxidative stress toxicity, metabolism, and protective effects of various compounds in vivo and in vitro Effects of nerol on paracetamol induced liver damage in Wistar albino rats ScienceDirect Oxidative stress mediates end organ damage in a novel model of acetaminophen toxicity in Drosophila Scientific Reports